The present invention relates to a hydrophobic and antifouling eco-friendly floor mat for livestock manure in livestock barns. More specifically, the invention provides a mat woven using natural fibers and installed on the floor surface inside a livestock barn, whereby feces and urine are naturally separated when livestock excrete manure, thus enabling the maintenance of a dry barn environment. In addition, through a surface structure in which grooves are formed in the mat, the contact area of the feces with the mat is minimized, making drying easier and removal simple, thereby facilitating the treatment and utilization of livestock manure and increasing its added value.